Culturing, Storage, and Quantification of Rotaviruses
نویسندگان
چکیده
منابع مشابه
آفات انباری غلات و طرق مبارزه با آنها storage fests of grain and their control
چکیده ندارد.
15 صفحه اولQuantification of the effect of culturing temperature on salt-induced heat resistance of bacillus species.
Short- and long-term exposure to mild stress conditions can activate stress adaptation mechanisms in pathogens, resulting in a protective effect toward otherwise lethal stresses. The mesophilic strains Bacillus cereus ATCC 14579 and ATCC 10987 and the psychrotolerant strain B. weihenstephanensis KBAB4 were cultured at 12 degrees C and 30 degrees C until the exponential growth phase (i) in the a...
متن کاملOvine rotaviruses
Rotavirus has been recognized as a predominant cause of acute diarrhea in young animals and humans. Rotavirus has segmented genome composed of 11 segments of double stranded RNA. The virus has a triple layered protein shell consisting of a core, an inner capsid and an outer capsid. The inner capsid protein is responsible for group specificity and based on it rotaviruses are classified into seve...
متن کاملDNA quantification of basidiomycetous fungi during storage of logging residues
The demand for bioenergy caused an increased use of logging residues, branches and treetops that were previously left on the ground after harvesting. Residues are stored outdoors in piles and it is unclear to what extent fungi transform this material. Our objective was to quantify the amount of wood degrading fungi during storage using quantitative real-time PCR (qPCR) to detect basidiomycetous...
متن کاملIsolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses.
Seven rotavirus strains were isolated in cell cultures from the intestinal contents of piglets with diarrhea. MA104 cells with pancreatin in the cell culture medium was the host system of choice for virus isolation and replication. A cell culture immunofluorescence test in which MA104 cells were used in microtiter plates was very effective for detecting and assaying rotaviruses. A plaque reduct...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Protocols in Microbiology
سال: 2009
ISSN: 1934-8525,1934-8533
DOI: 10.1002/9780471729259.mc15c03s15